oleh Najla Alyaa' binti Mohd Nazri dan Nurul Hanim Azreen binti Zulkifly

The practicum at UPM-MAKNA Cancer Research Laboratory (CANRES), Institute of Bioscience (IBS) was an immersive experience in conducting cytotoxic assays and cancer cell research, allowing us to develop extensive hands-on expertise with advanced laboratory techniques. We began our practicum journey by reporting to our supervisor, Mr. Rusdam and we were introduced to the specialized laboratory areas and equipment. Our initial tasks included identifying cancer cell morphology with an inverted microscope and learning about migration and clonogenic assays. We were also trained in maintaining sterile techniques and handling sensitive cell cultures, both essential for ensuring reliable experimental outcomes

During the practicum, we gained practical skills in key laboratory procedures, including media changes, cell thawing, cryopreservation, and seeding. We assessed cell toxicity in MCF7 cells, performed MTT assays to measure viability, and conducted ELISA readings to validate results. These hands-on tasks were complemented by theoretical insights from reading materials and guidance from laboratory supervisors, enhancing our understanding of the complexities of cancer research.

In the later stages of the practicum, we focused on verification processes, such as temperature checks for laboratory equipment and micropipette calibration, ensuring accuracy in our experiments. We also prepared reports and articles that reflected on our experiences, synthesizing our knowledge of virotherapy under the mentorship of Dr. Eddie. This period allowed us to integrate practical skills with theoretical knowledge, deepening our expertise in a professional setting.

Beyond our laboratory work, we participated in community-building activities, including a ‘gotong-royong’ for planting herbs and the ‘Semarak Merdeka’ competition to make the national pastry, ‘bahulu’. These activities not only fostered teamwork but also provided us with a glimpse into the institute’s vibrant cultural environment. The combination of demanding scientific tasks and engaging social events offered a well-rounded perspective of the IBS environment, underscoring the importance of both precision in research and the value of teamwork.

Figure 1 One of the procedures involved in cell thawing		Figure 2 Celebration of ‘Semarak Merdeka’ at IBS UPM
Figure 3 Activity of ‘gotong - royong’ at the herb garden together with the CanRes lab staff		Figure 4 The appreciation certificate to the CanRes lab

Date of Input: 30/09/2024 | Updated: 30/09/2024 | azah